Product: Oat Beta Glucan Mw Individual Standard
Structure description: Linear chain with mixed linkage of (1,3) and (1,4);
The ratio of tri- and tetra- building block is ~2:1
Properties:
¡¡¡¡¡¡Appearance: white powder, odorless
¡¡¡¡¡¡Starch: not detected (n.d.)
Protein: n.d.
Moisture: < 3%
Arabinoxylan: < 0.5%
Ash: < 0.5%
Beta-glucan: > 97%
Water-solubility: water soluble.
Molecular weight (Mw)and Polydispersity (Pd):
| Standards | PS-OBG -10 |
PS-OBG -20 |
PS-OBG -30 |
PS-OBG -50 |
PS-OBG -80 |
PS-OBG -130 |
PS-OBG -160 |
| Mw X 10-4 | 1.1 | 2.2 | 3.1 | 5.2 | 8.1 | 13.09 | 16.06 |
| Pd | 1.02 | 1.02 | 1.03 | 1.03 | 1.03 | 1.03 | 1.03 |
| Standards | PS-OBG-200 | PS-OBG-270 | PS-OBG-300 | PS-OBG-500 | PS-OBG-700 | PS-OBG-900 |
|
| Mw X 10-4 | 19.06 | 26.5 | 29.0 | 50.0 | 72.0 | 89.0 |
|
| Pd | 1.03 | 1.03 | 1.05 | 1.05 | 1.05 | 1.05 |
|
Weight average molecular weight Mw was determined by Viscotek triple detector array system equipped
with size exclusion chromatography, light scattering detector using a 90o angle geometry;
dn / dc = 0.146
Method of dissolution:
1. Measure a specified amount of standards in a sample bottle;
2. Add distilled water and dissolve it at 60¡æfor 15-30minutes, stir the water gently until they dissolve homegeneously with the water;
3. Filtrate the solution with a 0.45 ¦̀m filter and a standard solution will be obtained.
Storage:
1. Store dry at room temperature in well sealed containers. Under these conditions, the product is stable for several years.
2. When in a liquid state, keep it in a refrigerator at 4¡æ. If filtrated with a 0.45 ¦̀m filter and added 0.02 wt% sodium azide as an antiseptic, it can be preserved at 4¡æ for 2 months.
.