Product: Putus oat beta-glucan General Mw standard kit
Structure description: Linear chain with mixed linkage of (1,3) and (1,4);
The ratio of tri- and tetra- building blocks is ~2:1
Properties:
Appearance: white powder, odorless
Starch: not detected (n.d.)
Protein: n.d.
Moisture: < 3%
Arabinoxylan: < 0.5%
Ash: < 0.5%
Beta-glucan: > 97%
Water-solubility: water soluble.
Molecular weight (Mw)and Polydispersity (Pd):
| Standards | PS-OBG- 10 |
PS-OBG- 30 |
PS-OBG- 80 |
PS-OBG- 200 |
PS-OBG- 300 | PS-OBG- 500 |
PS-OBG- 700 |
PS-OBG- 900 |
|
Mw X10-4 |
1.1 | 3.1 | 8.1 | 19.06 | 29.0 | 50.0 | 72.0 | 89.0 |
| Pd | 1.02 | 1.03 | 1.03 | 1.03 | 1.05 | 1.05 | 1.05 | 1.05 |
Molecular weights were determined by Viscotek triple detector array system including size exclusion chromatography, refractive index detector and light scattering detector using a 90o angle geometry. dn/dc = 0.146
Figure The size exclusion chromatography profile of PS-OBG-GMw kit.
These curves from left to right represent PS-OBG-900 to PS-OBG-10 respectively.
Method of dissolution:
1. Measure a specified amount of standards in a sample bottle;
2. Add distilled water and dissolve it at 60¡æ for 15-30minutes, stir the water gently until they dissolve homegeneously with the water;
3. Filtrate the solution with a 0.45 ¦̀m filter and a standard solution will be obtained.
Storage:
1. Store dry at room temperature in well sealed containers. Under these conditions, the product is stable for several years
2. When in a liquid state, keep it in a refrigerator at 4¡æ. If filtrated with a 0.45 ¦̀m filter and added 0.02 wt% sodium azide as an antiseptic, it can be preserved at 4¡æ for 2 months.
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